Short Description

This test is useful for validation of the reported variants where testing is performed using NGS technology for the proband and all the additional family members. In addition sanger sequencing can be performed for all the major hotspots, SNPs or any rare variant within human genome.

PreTest Information

Duly filled Sanger Sequencing Test Requisition Form (Form 38) is mandatory.

Detail Description

Sanger Sequencing: Single Variant Test

At DNA Labs India, we offer Sanger sequencing for single variant testing at an affordable cost of INR 14,000. This test is designed to identify specific genetic variations in an individual's DNA.

Symptoms and Diagnosis

Sanger sequencing for single variant testing is recommended for individuals who are suspected to have a genetic disorder or have a family history of a particular genetic condition. Symptoms of genetic disorders can vary widely, depending on the specific condition. Some genetic disorders may be apparent at birth, while others may not become apparent until later in life.

Diagnosis of a genetic disorder typically involves a comprehensive evaluation of an individual's medical history, family history, and physical examination. Genetic testing, including Sanger sequencing, may be recommended to confirm a diagnosis or to determine the presence of a genetic variation that may increase the risk of developing a particular condition.

How Sanger Sequencing Works

Sanger sequencing is a type of genetic testing that involves sequencing a small segment of DNA. This technique was first developed by Frederick Sanger in the 1970s and has since become a widely used method for genetic testing.

The process of Sanger sequencing involves several steps:

• Isolation of DNA from a sample, such as blood or saliva
• Amplification of a specific DNA segment using polymerase chain reaction (PCR)
• Sequencing of the amplified DNA using fluorescently labeled nucleotides
• Analysis of the sequence data to identify specific genetic variations

Benefits of Sanger Sequencing

Sanger sequencing is a highly accurate method for identifying genetic variations. It can detect even small changes in DNA sequence, making it an ideal method for identifying single nucleotide polymorphisms (SNPs) and other small variations.

Sanger sequencing is also relatively inexpensive compared to other genetic testing methods, making it an accessible option for many individuals.

Conclusion

Sanger sequencing for single variant testing is a valuable tool for identifying specific genetic variations that may be associated with a particular condition. At DNA Labs India, we offer this test at an affordable cost of INR 14,000. If you have any questions about Sanger sequencing or genetic testing in general, please don't hesitate to contact us.

This test is useful for validation of the reported variants where testing is performed using NGS technology for the proband and all the additional family members. In addition sanger sequencing can be performed for all the major hotspots, SNPs or any rare variant within human genome.