Short Description

Glutamine synthetase (GS) is an enzyme that catalyzes the ATP-dependent condensation of glutamate with ammonia to form glutamine. GS can be used with a panel of IHC markers like Beta-catenin, Liver fatty acid binding protein, C-reactive protein and Amyloid-A to distinguish Hepatic adenoma from Focal nodular hyperplasia and nonneoplastic liver. GS, a target gene of Beta-catenin, is expressed in Hepatic adenomas with Beta-catenin mutations (type 2), but is not expressed in Hepatic adenomas without Beta-catenin mutations. GS is expressed in zone 3 of normal liver and has a characteristic map-like pattern in Focal nodular hyperplasia.It is mainly used for the classification of Hepatic adenomas and the identification of Focal nodular hyperplasia.

PreTest Information

Provide a copy of the Histopathology report, Site of biopsy and Clinical history.

Detail Description

IMMUNOHISTOCHEMISTRY GLUTAMINE SYNTHETASE Test Cost INR:3500 Symptoms Diagnosis

Immunohistochemistry is a technique that is used to detect the presence and location of specific proteins in tissue samples. Glutamine synthetase is an enzyme that plays a key role in the metabolism of nitrogen and is commonly used as a marker in immunohistochemistry tests. This test is used to diagnose a variety of disorders, including liver disease, kidney disease, and brain tumors.

What is Glutamine Synthetase?

Glutamine synthetase is an enzyme that is found in almost all living organisms. It is responsible for catalyzing the conversion of glutamate to glutamine, which is an important amino acid that is involved in a range of metabolic processes. Glutamine synthetase is particularly abundant in the liver, kidneys, and brain, where it plays an important role in maintaining nitrogen balance and preventing the accumulation of toxic ammonia.

Why is the Immunohistochemistry Glutamine Synthetase Test Done?

The immunohistochemistry glutamine synthetase test is done to detect the presence and location of glutamine synthetase in tissue samples. This test is commonly used to diagnose a variety of disorders, including liver disease, kidney disease, and brain tumors. Some of the symptoms that may lead to a doctor ordering this test include:

• Abdominal pain
• Nausea and vomiting
• Jaundice
• Confusion or memory loss
• Headaches
• Seizures

How is the Immunohistochemistry Glutamine Synthetase Test Done?

The immunohistochemistry glutamine synthetase test is typically done on a tissue sample that has been removed during a biopsy or surgery. The tissue sample is first fixed in formalin and then embedded in paraffin wax. Thin slices of the tissue are then cut using a microtome and placed on glass slides. The slides are then treated with a primary antibody that is specific to glutamine synthetase. This antibody binds to the glutamine synthetase protein in the tissue sample, allowing it to be detected and located using a technique called immunofluorescence.

What is the Cost of the Immunohistochemistry Glutamine Synthetase Test?

The cost of the immunohistochemistry glutamine synthetase test in India is generally around INR 3500. However, the cost may vary depending on the location and the laboratory where the test is performed. It is always a good idea to check with the laboratory beforehand to confirm the cost of the test.

Conclusion

The immunohistochemistry glutamine synthetase test is a valuable diagnostic tool that can help doctors diagnose a range of disorders. By detecting the presence and location of glutamine synthetase in tissue samples, this test can provide important information about the underlying cause of a patient's symptoms. If you are experiencing any symptoms that may be related to liver disease, kidney disease, or brain tumors, it is important to speak with your doctor to determine if the immunohistochemistry glutamine synthetase test is right for you.

At DNA Labs India, we offer a wide range of diagnostic tests, including the immunohistochemistry glutamine synthetase test. Our experienced team of professionals is dedicated to providing accurate and reliable results in a timely manner. Contact us today to learn more about our services.

Glutamine synthetase (GS) is an enzyme that catalyzes the ATP-dependent condensation of glutamate with ammonia to form glutamine. GS can be used with a panel of IHC markers like Beta-catenin, Liver fatty acid binding protein, C-reactive protein and Amyloid-A to distinguish Hepatic adenoma from Focal nodular hyperplasia and nonneoplastic liver. GS, a target gene of Beta-catenin, is expressed in Hepatic adenomas with Beta-catenin mutations (type 2), but is not expressed in Hepatic adenomas without Beta-catenin mutations. GS is expressed in zone 3 of normal liver and has a characteristic map-like pattern in Focal nodular hyperplasia.It is mainly used for the classification of Hepatic adenomas and the identification of Focal nodular hyperplasia.