Short Description

DNA QC and quantitation using Nanodrop, preparation of 96 GBS libraries using ApeKI restriction enzyme, multiplexing of barcoded libraries and 2x150 PE Illumina Hiseq sequencing to generate total of 400 million reads (R1+R2) per library pool. Variant calling and genotyping. Data will be delivered as FASTQ sequences as well as genotype information in spreadsheet.

PreTest Information

Detail Description

Genotyping by sequencing and primary analysis-96 samples cost INR:450000 for DNA Labs India

Genotyping by sequencing (GBS) is a technique used to determine the genetic variation present in a particular organism. This technique involves the sequencing of a subset of the genome, usually the regions that contain genetic variation. The resulting data can be used for a variety of applications, including population genetics, plant and animal breeding, and disease research.

At DNA Labs India, we offer genotyping by sequencing services for 96 samples at a cost of INR 450000. This service includes primary analysis of the data, which involves quality control, alignment to a reference genome, and variant calling.

Symptoms and Diagnosis

Genotyping by sequencing can be used to diagnose genetic disorders and diseases. By comparing the genetic variation present in an individual's genome to known disease-causing mutations, doctors and researchers can identify the underlying cause of a patient's symptoms.

For example, genotyping by sequencing can be used to diagnose inherited diseases such as cystic fibrosis or sickle cell anemia. By sequencing the relevant genes, doctors can identify the specific mutations that are causing the disease and develop targeted treatments.

Genotyping by sequencing can also be used to diagnose infectious diseases. By sequencing the genome of a pathogen, researchers can identify the specific strain causing an outbreak and track its spread.

Benefits of Genotyping by Sequencing

Genotyping by sequencing offers several benefits over traditional genotyping techniques. For one, it allows for the identification of genetic variation across the entire genome, rather than just in specific regions. This makes it particularly useful for population genetics and breeding studies.

Genotyping by sequencing is also highly accurate and can detect low-frequency variants that may be missed by other techniques. This makes it particularly useful for disease research, where identifying rare variants can be critical to understanding the underlying biology of a disease.

Finally, genotyping by sequencing is highly scalable and can be used to analyze large numbers of samples simultaneously. This makes it particularly useful for large-scale studies and clinical trials.

Conclusion

Genotyping by sequencing is a powerful tool for understanding genetic variation and its role in disease. At DNA Labs India, we offer genotyping by sequencing services for 96 samples at a cost of INR 450000. This service includes primary analysis of the data, which involves quality control, alignment to a reference genome, and variant calling. If you are interested in learning more about our genotyping by sequencing services, please contact us today.

Thank you for considering DNA Labs India for your genotyping by sequencing needs.