Short Description

Eukaryotic rRNA depletion, stranded RNA Seq library preparation, 2x150 PE Sequencing using Illumina HiSeq to generate 50-60 million reads (R1+R2). Alignment of quality filtered reads onto reference genome, transcript abundance estimation, differential gene expression analysis and identification of statistically significant up/down regulated genes, functional annotation of significant genes (Gene Ontology and Pathway). Analysis covers mRNA as well as lncRNA.

PreTest Information

Detail Description

Eukaryotic Stranded Transcriptome Sequencing and Reference-Based Analysis

DNA Labs India offers Eukaryotic Stranded Transcriptome Sequencing and Reference-Based Analysis, including lncRNA at a cost of INR 50,000. This advanced technique is used to study the transcriptome of eukaryotic organisms and can provide valuable insights into gene expression, alternative splicing, and post-transcriptional regulation.

What is Eukaryotic Stranded Transcriptome Sequencing?

Eukaryotic Stranded Transcriptome Sequencing is a next-generation sequencing (NGS) technique that is used to sequence the entire transcriptome of eukaryotic organisms. This includes not only protein-coding genes but also non-coding RNAs such as long non-coding RNAs (lncRNAs). This technique is based on the capture and sequencing of RNA molecules that are transcribed from DNA and provides a comprehensive view of gene expression in a particular tissue or cell type.

What is Reference-Based Analysis?

Reference-Based Analysis is a bioinformatics technique that is used to compare the sequence data obtained from Eukaryotic Stranded Transcriptome Sequencing to a reference genome or transcriptome. This analysis allows for the identification of differentially expressed genes, alternative splicing events, and post-transcriptional modifications. It can also be used to identify novel transcripts that have not previously been annotated.

What are lncRNAs?

Long non-coding RNAs (lncRNAs) are a class of non-coding RNA molecules that are longer than 200 nucleotides. They are transcribed from DNA but do not code for proteins. Instead, they play important roles in the regulation of gene expression, chromatin structure, and epigenetic modifications. lncRNAs have been implicated in a wide range of biological processes including development, differentiation, and disease.

Why is Eukaryotic Stranded Transcriptome Sequencing and Reference-Based Analysis Important?

Eukaryotic Stranded Transcriptome Sequencing and Reference-Based Analysis are important tools for studying gene expression and regulation in eukaryotic organisms. This technique can provide valuable insights into the molecular mechanisms underlying cellular processes and can help researchers to identify potential therapeutic targets for diseases such as cancer and neurological disorders. Additionally, the identification of novel transcripts such as lncRNAs can provide new avenues for further research and discovery.

What are the Symptoms and Diagnosis?

Eukaryotic Stranded Transcriptome Sequencing and Reference-Based Analysis are not diagnostic tests. They are research tools that are used to study gene expression and regulation in eukaryotic organisms. However, the information obtained from these analyses can be used to gain a better understanding of the molecular mechanisms underlying disease processes and to identify potential therapeutic targets.

Conclusion

Eukaryotic Stranded Transcriptome Sequencing and Reference-Based Analysis, including lncRNA, is a powerful tool for studying gene expression and regulation in eukaryotic organisms. This technique can provide valuable insights into the molecular mechanisms underlying cellular processes and can help researchers to identify potential therapeutic targets for a wide range of diseases. DNA Labs India offers this advanced technique at a cost of INR 50,000.

If you are interested in learning more about Eukaryotic Stranded Transcriptome Sequencing and Reference-Based Analysis, please contact DNA Labs India today.