Short Description

DNA QC and quantitation by Nanodrop and/or Agarose Gel Electrophoresis.

PreTest Information

Detail Description

DNA QC and Quantitation -Nanodrop cost INR:200 symptoms diagnosis

DNA quality control (QC) and quantitation are crucial steps in any molecular biology experiment. It involves the assessment of DNA sample purity and concentration, which is essential for downstream applications such as PCR, sequencing, and cloning. Nanodrop is a commonly used instrument for DNA QC and quantitation, and its cost in India is around INR 200.

Symptoms of poor DNA quality

Poor DNA quality can lead to inaccurate results and failure of downstream applications. The symptoms of poor DNA quality include:

• Low yield
• Low purity
• Degraded DNA
• Contaminants such as proteins, RNA, and chemicals

These symptoms can be caused by various factors such as improper sample preparation, storage, and handling.

Diagnosis of DNA quality

There are several methods to diagnose DNA quality, including:

• Agarose gel electrophoresis
• Spectrophotometry
• Fluorometry

Agarose gel electrophoresis is a traditional method that separates DNA fragments based on size and provides information on DNA integrity. However, it is time-consuming and requires specialized equipment. Spectrophotometry and fluorometry are more commonly used methods that measure the absorbance or fluorescence of DNA samples and provide information on DNA concentration and purity.

Nanodrop for DNA QC and quantitation

Nanodrop is a spectrophotometer that is widely used for DNA QC and quantitation due to its high sensitivity and speed. It uses a small sample volume (typically 1-2 µl) and measures the absorbance at multiple wavelengths to determine DNA concentration and purity. The purity of DNA is calculated by the ratio of absorbance at 260 nm and 280 nm (A260/A280), where a ratio of 1.8 is considered pure DNA.

Nanodrop is a cost-effective option for DNA QC and quantitation, with a cost of around INR 200 in India. However, it has some limitations such as interference from contaminants and inaccurate results for low-concentration samples. Therefore, it is important to use Nanodrop in conjunction with other methods such as agarose gel electrophoresis to ensure accurate and reliable results.

Conclusion

DNA QC and quantitation are critical steps in any molecular biology experiment, and Nanodrop is a commonly used instrument for this purpose. Its cost in India is around INR 200, making it a cost-effective option for researchers. However, it is important to use Nanodrop in conjunction with other methods to ensure accurate and reliable results.

By taking the necessary steps to ensure high-quality DNA, researchers can avoid inaccurate results and achieve successful downstream applications.