Cell Line Authentication DNA Test
Disease: Genetics
Method: STR DNA Technology
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Cell Line Authentication DNA Test Cost 6500 Rs
Cell Line Authentication DNA Test Details
Frequently Asked Questions
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What is the cost of Cell Line Authentication DNA Test?
Cost of Cell Line Authentication DNA Test is 6500 Rs
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What is Cell Line Authentication?
Tissue culture cells are important tools in many laboratories. Unfortunately, many cell lines are misidentified or contaminated with other cells, potentially invalidating published data and wasting time and laboratory resources.
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When should cell line authentication be conducted?
Authentication is required every time a new line is established or acquired. Cells should be authenticated again before freezing, once every two months that the culture is actively growing, at any point that the performance of the line is inconsistent or yields unexpected results, and before publication.
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How does it work?
The number of repeats in STR loci can be highly variable among individuals, which make these genetic markers effective for human identification or cell line authentication purposes. STRs have become popular DNA repeat markers because they are easily amplified by the polymerase chain reaction (PCR) using PCR primers that bind to the flanking regions surrounding the STR repeat.
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Cell Line Authentication and Characterization Methods
Cell line characterization is performed for Master Cell Banks (MCB), Working Cell Banks (WCB), and End-of-Production Cells (EPC) or cells at the limit (CAL) of in vitro cell age used for production. According to current regulations, the following aspects must be considered:
- The origin and history of the cell line cellular morphology and growth characteristics
- Cellular identity Purity of the cell lines (i.e., absence of contaminating cells, microbial contaminations, and contaminations by adventitious viruses)
- Tumorigenicity and oncogenicity Stability
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Cell Line Characterization
Proper cell line characterization assays are crucial to confirm the certainty of contaminant-free cell banks. Extensive cell line authentication, including phenotypic or genotypic identity testing, is required for mammalian, microbial, and insect cell substrates testing when working to generate biopharmaceuticals.
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Authentication of Nonhuman Cell Lines
When considering authentication of nonhuman cell lines, the extensive body of technical knowledge on STR profiling, which is captured in the ANSI standard and by other organizations (e.g., The International Society for Forensic Genetics), and the proven record of practicality and fit-for-purpose of STR profiling, make it an obvious choice for consideration as a standard method for nonhuman cell lines.
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What is the cost of Cell Line Authentication?
The cost of Cell Line Authentication is 6500 Rs
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How many days does it take for reports?
Reports will take 8-10 working days