Short Description

Enrichment of 16S rRNA V3-V4 hyper variable region and preparation of sequencing libraries with Illumina sequencing adapters. 2x300 PE Sequencing using Illumina Miseq to generate minimum 0.1 Million reads (R1+R2) per library.

PreTest Information

Detail Description

Bacterial V3-V4 16S rRNA Amplicon Sequencing Cost INR:7000 Symptoms Diagnosis

Are you experiencing symptoms of a bacterial infection? Have you been advised to undergo bacterial V3-V4 16S rRNA amplicon sequencing for diagnosis? DNA Labs India offers this test at an affordable cost of INR 7000. Read on to learn more about this test and how it can help in diagnosis.

What is Bacterial V3-V4 16S rRNA Amplicon Sequencing?

Bacterial V3-V4 16S rRNA amplicon sequencing is a molecular diagnostic test that uses DNA sequencing technology to identify bacterial species in a patient's sample. The V3-V4 region of the bacterial 16S rRNA gene is targeted for amplification and sequencing. This region is highly conserved among bacteria and is therefore useful for identifying bacterial species.

Why is Bacterial V3-V4 16S rRNA Amplicon Sequencing Done?

Bacterial V3-V4 16S rRNA amplicon sequencing is done to diagnose bacterial infections in patients. This test is particularly useful when conventional microbiological methods fail to identify the causative agent of an infection. It is also useful in cases where the infection is caused by an uncommon or fastidious bacterial species that is difficult to culture.

What are the Symptoms of a Bacterial Infection?

The symptoms of a bacterial infection can vary depending on the type and severity of the infection. Some common symptoms of a bacterial infection include:

• Fever
• Chills
• Fatigue
• Body aches
• Nausea
• Vomiting
• Diarrhea
• Headache
• Sore throat
• Cough
• Difficulty breathing

How is Bacterial V3-V4 16S rRNA Amplicon Sequencing Done?

Bacterial V3-V4 16S rRNA amplicon sequencing is done on a patient's sample, which can be blood, urine, stool, or any other body fluid or tissue. The sample is processed in the laboratory to extract bacterial DNA, which is then amplified using PCR (polymerase chain reaction) to generate a large number of copies of the V3-V4 region of the 16S rRNA gene. The amplified DNA is then sequenced using next-generation sequencing technology, and the resulting sequences are compared to a database of known bacterial species to identify the causative agent of the infection.

What is the Cost of Bacterial V3-V4 16S rRNA Amplicon Sequencing?

The cost of bacterial V3-V4 16S rRNA amplicon sequencing at DNA Labs India is INR 7000.

Conclusion

Bacterial V3-V4 16S rRNA amplicon sequencing is a valuable tool in the diagnosis of bacterial infections. This test can identify bacterial species that are difficult or impossible to culture using conventional methods. DNA Labs India offers this test at an affordable cost of INR 7000. If you are experiencing symptoms of a bacterial infection and have been advised to undergo this test, do not hesitate to contact DNA Labs India for accurate and timely diagnosis.