Short Description

Bacterial rRNA depletion, RNA Seq library preparation, 2x150 PE sequencing using Illumina Hiseq to generate 10 million reads (R1+R2). De novo transcriptome assembly optimisation and assembly of transcriptome, clustering and scaffolding, assembly quality check, transcriptome annotation. Gene expression analysis by mapping sequence reads onto the annotated transcriptome, differential gene expression analysis, functional enrichment analysis (Gene Ontology and Pathway enrichment).

PreTest Information

Detail Description

Bacterial Transcriptome Sequencing and De Novo Analysis Cost in India

Bacterial infections can cause a wide range of symptoms, from mild discomfort to severe illness. Identifying the specific bacteria causing the infection is essential for effective treatment. DNA Labs India offers bacterial transcriptome sequencing and de novo analysis to help diagnose bacterial infections.

What is Bacterial Transcriptome Sequencing?

Bacterial transcriptome sequencing is a technique used to identify the genes that are actively expressed in a bacterial cell. By analyzing the bacterial transcriptome, it is possible to gain insight into the biological processes that are occurring within the cell, including the production of virulence factors and antibiotic resistance mechanisms.

What is De Novo Analysis?

De novo analysis is a process of assembling and analyzing genomic data without the use of a reference genome. In the case of bacterial transcriptome sequencing, de novo analysis can be used to identify novel genes and gene variants that may be important for virulence or antibiotic resistance.

How is Bacterial Transcriptome Sequencing and De Novo Analysis Performed?

Bacterial transcriptome sequencing and de novo analysis involve several steps:

• Isolation of bacterial RNA
• Conversion of RNA to cDNA
• Sequencing of cDNA using next-generation sequencing technology
• Assembly of transcriptome data using de novo analysis software
• Annotation of assembled transcripts to identify genes and gene variants

What are the Symptoms of Bacterial Infections?

The symptoms of bacterial infections can vary depending on the type of bacteria involved and the location of the infection. Common symptoms of bacterial infections include:

• Fever
• Pain or swelling at the site of infection
• Redness or warmth at the site of infection
• Difficulty breathing
• Nausea and vomiting
• Diarrhea
• Joint pain

How are Bacterial Infections Diagnosed?

Bacterial infections can be diagnosed through a variety of methods, including:

• Physical examination
• Blood tests
• Urinalysis
• Imaging tests
• Culture of bacteria from a sample
• Bacterial transcriptome sequencing and de novo analysis

What is the Cost of Bacterial Transcriptome Sequencing and De Novo Analysis?

The cost of bacterial transcriptome sequencing and de novo analysis at DNA Labs India is INR 30,000. This cost includes the isolation of bacterial RNA, conversion of RNA to cDNA, sequencing of cDNA using next-generation sequencing technology, assembly of transcriptome data using de novo analysis software, and annotation of assembled transcripts to identify genes and gene variants.

Conclusion

Bacterial transcriptome sequencing and de novo analysis can provide valuable information for the diagnosis and treatment of bacterial infections. DNA Labs India offers this service at an affordable cost of INR 30,000. If you are experiencing symptoms of a bacterial infection, it is important to seek medical attention and discuss the possibility of bacterial transcriptome sequencing and de novo analysis with your healthcare provider.

Contact DNA Labs India to learn more about our bacterial transcriptome sequencing and de novo analysis services.