Short Description

Enrichment of 16S rRNA V3-V4 hyper variable region and preparation of sequencing libraries with Illumina sequencing adapters. 2x300 PE Sequencing using Illumina Miseq to generate minimum 0.1 Million reads (R1+R2) per library. Metagenome analysis for OTU assignment, abundance (alpha diversity), beta diversity analysis and data visualisation (rarefaction curve, stacked bar plots, heatmaps, PCOA plots).

PreTest Information

Detail Description

Bacterial 16S rRNA V3-V4 Amplicon Sequencing and Analysis Cost INR 10,000: Symptoms and Diagnosis

When it comes to identifying bacterial species, there are various methods available in the market. One of the most reliable techniques is the 16S rRNA gene sequencing. It is a molecular technique that helps in identifying different bacterial species and understanding their phylogenetic relationships.

Amongst the different regions of the 16S rRNA gene, the V3-V4 region is one of the most commonly used regions for bacterial identification. The reason being, it has a high degree of variability and can help in distinguishing between closely related bacterial species.

Bacterial 16S rRNA V3-V4 Amplicon Sequencing and Analysis Cost

The cost of bacterial 16S rRNA V3-V4 amplicon sequencing and analysis varies depending upon the service provider and the number of samples to be analyzed. However, the average cost for this service in India is around INR 10,000 per sample.

The cost may seem high, but it is a reliable and accurate technique that can help in identifying bacterial species that may be missed through traditional culture-based methods. Furthermore, the information obtained from this technique can help in understanding the bacterial community present in a particular sample.

Symptoms and Diagnosis

Bacterial infections can cause a wide range of symptoms, ranging from mild to severe. Some of the common symptoms of bacterial infections include:

• Fever
• Chills
• Fatigue
• Pain or discomfort in the affected area
• Nausea and vomiting
• Diarrhea
• Cough
• Sore throat

However, these symptoms can be caused by various other factors as well. Therefore, it is essential to get a proper diagnosis to determine the underlying cause of the symptoms.

Bacterial 16S rRNA V3-V4 amplicon sequencing and analysis can help in the diagnosis of bacterial infections. The technique involves obtaining a sample from the site of infection and analyzing the bacterial DNA present in the sample. The information obtained from the analysis can help in identifying the bacterial species causing the infection and determining the appropriate treatment.

Conclusion

Bacterial 16S rRNA V3-V4 amplicon sequencing and analysis is a reliable and accurate technique that can help in identifying bacterial species and understanding their phylogenetic relationships. The cost of this service in India is around INR 10,000 per sample. This technique can be beneficial in the diagnosis of bacterial infections, which can cause a wide range of symptoms.

If you suspect that you have a bacterial infection, it is essential to seek medical attention and get a proper diagnosis. Bacterial 16S rRNA V3-V4 amplicon sequencing and analysis can be a valuable tool in the diagnosis and treatment of bacterial infections.